· The polymerase on which SsoAdvanced fused with Sso7d was based was also not reported, and the length of the main amplification product is 1 bp longer than that of Taq polymerase (Fig. 1 and Table 4). It is considered to not have proofreading activity because it is a real-time PCR reagent and such activity is not described in the manual. iProof™ High-Fidelity DNA Polymerase. iProof™ High-Fidelity DNA Polymerase. Published on January | Categories: Documents | Downloads: 67 | Comments: 0. iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.. Features and Benefits of iProof High-Fidelity DNA Polymerase: High fidelity — iProof DNA polymerase is fold more accurate than Taq polymerase.
EPA1 EPA EPA EPA1 EP A1 EP A1 EP A1 EP A EP A EP A EP A1 EP A1 EP A1 Authority. Fidelity measurements of Taq DNA Polymerase (in Standard Taq Buffer), KAPA HiFi HotStart ReadyMix and NEBNext High-Fidelity 2X PCR Master Mix were measured side-by-side in a PCR-based mutation screening assay using a lacZ method modified from Kermekchiev et al., Values (n ≥ 2) are expressed relative to KAPA HiFi HotStart ReadyMix. iProof™ High-Fidelity DNA Polymerase. iProof™ High-Fidelity DNA Polymerase. Published on January | Categories: Documents | Downloads: 67 | Comments: 0.
High fidelity — iProof DNA polymerase is fold more accurate than Taq polymerase Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (–1 unit/reaction). for PCR. High fidelity iProof DNA polymerase is fold more earnest than Taq polymerase Speed high processivity dramatically reduces extension seckb. In medical diagnostics, or denature the phusion losing its strands identical to taq polymerase. Takara Taq products Takara Taq and premix Takara Taq PCR kit High-yield PCR Titanium Taq products. iProof™ High-Fidelity PCR Kit 2 units/µl, 25 µl 50U 2 units/µl, µl U For research purposes only Store at °C iProof is a high-fidelity DNA polymerase that offers extreme performance for all PCR applications. Incorporating an exciting new and patented technology, iProof DNA.
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